NUCLEOTIDE SEQUENCES WHICH CODE FOR THE METR AND METZ GENES

摘要

The invention relates to polynucleotides from coryneform bacteria which code for the metR and/or metZ genes and comprise polynucleotide sequences, chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 3, c) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, d) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 3, e) polynucleotide which is complementary to the polynucleotides of a) b), c) or d), and f) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequences of a), b), c), d) or e) and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metR gene and/or the metZ gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.