Verfahren zur Reduktion des Liganden-leakage von Affinitätschromatographie-Matrices

摘要

The invention relates to a method for (pre)treating affinity chromatography matrices, preferably protein A matrices, comprising at least one surface active substance for reducing ligand leakage. Treating the affinity chromatography matrix according to the invention makes it possible to obtain a constant quality of the affinity chromatography matrix thereby representing an important requirement for the suitability/use in biopharmaceutical processes. The invention also relates to methods for determining the ligand leakage.