| 摘要 |
FIELD: organic chemistry, biotechnology, microbiology. SUBSTANCE: invention relates to synthesis of alpha-hydroxyacids and involves, in part, preparing alpha- hydroxyacids represented by the general formula [II]: RCH(OH)COOH where R means hydrogen atom, optionally substituted C1-C6-alkyl, optionally substituted C2-C6-alkenyl, optionally substituted C1-C6-alkoxyl, optionally substituted aryl, optionally substituted arylhydroxy-group or optionally substituted heterocycle as result of action of microorganism on alpha-hydroxynitriles of the formula [I]: RCH(OH)CN (where R is given above). The process involves hydrolysis and conversion of alpha-hydroxynitriles to alpha- hydroxyacids of the formula [II] and alpha-hydroxyacids [II] are accumulated in an aqueous solution. Microorganism showing concentration resistance to alpha-hydroxynitriles [I] and/or alpha-hydroxyacids [II] and stability in the presence of cyanide is used as microorganism. Then the end product is isolated from reaction medium. Variovarax spp., Arthrobacter spp. and especially the strain Arthrobacter NSSC 104 are used as the above mentioned microorganisms exhibiting concentration resistance to alpha-hydroxynitriles [I] and alpha-hydroxyacids [II] and sufficiently high stability. This gives a possibility to accumulate alpha- hydroxyacids [II] in high concentration, i. e. to provide their effective preparing. Addition of cyanide to reaction system providing one of variant of method results to the more effective preparing alpha- hydroxyacids [II]. EFFECT: improved preparing method. 15 cl, 4 tbl |
