COMPUTER PROGRAM FOR SIMULATING GENE CLONING PROCESS WITH SAME AS ACTUAL EXPERIMENTAL PROCESS AND STORING RESULT IN DATABASE, AND THE METHOD

摘要

PURPOSE: A computer program for simulating a gene cloning process with the same as an actual experimental process and storing a result in a database, and a method for the same are provided to enable all persons specialized in molecular biology to easily and conveniently use, store, and manage the precious information. CONSTITUTION: A primer designer module designs and makes a primer in order to perform the DNA(Deoxyribo Nucleic Acid) PCR(Polymerize Chain Reaction). A PCR module makes the PCR product by checking an annealing state between the primer and a sequence. A DNA cutter module makes the DNA product generated when the sequence is cut by a restriction enzyme. A ligation module makes one sequence through the addition of two sequences by calculating overhang of the two sequences. A cut and ligation module realizes all cloning processes at one time by inputting a vector sequence object, an insert sequence object, and the restriction enzyme necessary for cloning. A sequence editor module edits the sequence in order to fit to a sequence object expression equation used by a cloning editor module. A sequence database stores the sequence object making all data generated by each process and inputted products as the object.