LYSOSOMAL PROTEINS PRODUCED IN THE MILK OF NONHUMAN TRANSGENIC ANIMALS
摘要
<p>The invention provides transgenic nonhuman mammals producing phosphorylated lysosomal proteins in their milk, and methods of generating the same. Phosphorylation occurs at the 6' position of a mannose side chain residue. Also provided are methods of purifying lysosomal proteins from milk, and incorporating the proteins into pharmaceutical compositions for use in enzyme replacement therapy.</p>