AFFINITY SELECTED SIGNATURE PEPTIDES FOR PROTEIN IDENTIFICATION AND QUANTIFICATION
摘要
A method for protein identification in complex mixtures that utilizes affinity selection of constituent proteolytic peptide fragments unique to a protein analyte. These "signature peptides" function as analytical surrogates. Mass spectrometric analysis of the proteolyzed mixture permits identification of a protein in a complex sample without purifying the protein or obtaining its composite peptide signature.
申请公布号
EP1311858(A2)
申请公布日期
2003.05.21
申请号
EP20010932999
申请日期
2001.05.04
申请人
PURDUE RESEARCH FOUNDATION;REGNIER, FRED E.;CHAKRABORTY, ASISH B.;DORMADY, SHELLY J.;G'ENG, MINGHUI;JI, JUNYAN;RIGGS, LARRY D.;SIOMA, CATHY S.;WANG, SHIHONG;ZHANG, XIANG