| 摘要 |
The present invention is directed toward a polynucleotide synthesis support for use in automated polynucleotide synthesis of 3'-nitrogen functionalized polynucleotides. The invention includes, in one aspect, a polynucleotide synthesis reagent having structure (I), where T is an acid-cleavable hydroxy l protecting group; Q is a linker connecting the nitrogen and oxygen; R1 is a nitrogen substituent; R2 through R4 are separately hydrogen or lower alkyl; Y is an atom which is electronegative with respect to carbon; X1 is an atom which is electronegative with respect to carbon; Z is a bond or spacer arm; and W is a derivatized solid synthesis support capable of linking to Z. In a second aspect, the present invention includes a polynucleotide synthesis reagent having structure (II), where T is an acid-cleavable hydroxyl protecting group; Q is a linker connecting the nitrogen and oxygen; R1 is a nitrogen substituent; R2 through R4 are separately hydrogen or lower alkyl; Y is an atom which is electronegative with respect to carbon; X2 is an atom which is electronegative with respect to carbon; Z is bond or spacer arm; an d W is a derivatized solid synthesis support capable of linking to Z.
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