BIOTIN OPERON

摘要

A DNA sequence of a high biotin operon-expression system usable for breeding a bacterium having excellent biotin productivity is provided. The invention relates to a DNA sequence of the biotin operon characterized by having at least one base pair of either a nucleotide sequence of the regulatory region of the biotin operon of Escherichia coli or a nucleotide sequence in the vicinity of the bioB initiating codon which is mutated in comparison with the wild type strain. Escherichia coli transformed with a recombinant plasmid carrying such a DNA sequence has high biotin productivity.