FLUORESCENCE QUENCHING USED TO DETECT LIGATE/LIGAND ASSOCIATION EVENTS IN ELECTRICAL FIELDS

摘要

The invention relates to a method for detecting ligate/ligand association events by fluorescence quenching, which comprises as a first step the provision of a modified surface. The modification of the surface consists in the binding of at least one type of modified ligates, wherein said ligates are modified by at least one type of fluorophore bound to it. The further steps of the inventive method are: providing a sample that includes the ligands, applying an electrical field and adjusting a defined strength of the electrical field on the site of the modified ligates, contacting the sample with the modified surface, detecting the fluorescence of the fluorophore and comparing the detected fluorescence intensities with reference values.