| 摘要 |
<p>The present application relates to a functional assay for high-throughput screening and identification of plant genes involved in xylogenesis and secondary cell wall formation. The assay involves transforming Zinnia mesophyll cells in vitro under non-inducing or transdifferentiation-inducing conditions with DNA constructs comprising one or more polynucleotide sequences to a be tested and comparing the expression of the sequences in the non-induced and transdifferentiating cells. The assay is particularly useful for identifying promoters that are active in xylem and xylem-forming tissues and their transcriptional regulators.</p> |
