| 摘要 |
<p>An in vitro system for culturing whole virus has been established using molecular biology and gene recombinant technology, the intact HCV proliferating virus can be produced by the system. The whole genome containing 98 nucleotide behind 3' PolyA can be amplified from the serum of the patient of HEPATITIS C in the system. The method comprises: the NS5A and NA5B of the HCV genome are site-directed mutated; the expressing cassette of mark gene IRES-GFP is inserted in the NS5B 3' end of mutant HCV genome; cell is transfected with it; the progeny HCV virus of infective activity is obtained by culture.</p> |
