发明名称 |
Process for producing cis-3-hydroxy-L-proline |
摘要 |
An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.
|
申请公布号 |
US2003092153(A1) |
申请公布日期 |
2003.05.15 |
申请号 |
US20020138632 |
申请日期 |
2002.05.06 |
申请人 |
OZAKI AKIO;MORI HIDEO;SHIBASAKI TAKESHI;ANDO KATSUHIKO;OCHIAI KEIKO;CHIBA SHIGERU;UOSAKI YOICHI |
发明人 |
OZAKI AKIO;MORI HIDEO;SHIBASAKI TAKESHI;ANDO KATSUHIKO;OCHIAI KEIKO;CHIBA SHIGERU;UOSAKI YOICHI |
分类号 |
C12N9/02;C12P13/24;(IPC1-7):C12N9/06;C12Q1/68;C07H21/04;C12P21/02;C12N5/06 |
主分类号 |
C12N9/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|