| 摘要 |
The present invention relates to a method and a device for determining a concentration of a biological active substance in a sample by the means of an enzyme-linked immunosorbent assay ELISA. The device comprises a solid support within a tubing 17 for binding an immunosorbent and an inlet 18, 19 for fluids, a detector 15 for detecting radiation due to an activity in said tubing 17, wherein said tubing 17 is arranged inside a microchip 10 extending substantially in one plane, for conducting the fluids along the plane of the microchip 10. Said tubing 17 forms a reaction cell having a large detection area 22. The reaction cell of the microchip 10 is arranged perpendicular to the detector 15.The method comprises the steps of introducing the fluids into the tubing 17, conducting the fluids through the tubing 17 forming a reaction cell, in which reaction cell the radiation emitting activity takes place, and detecting the light emitted from the reaction cell substantially perpendicular to the plane. |
