Compositions and methods for production of recombinant viruses, and uses therefor

摘要

An efficient method of producing recombinant virus is described. The method involves transfecting an uncut, circular plasmid containing a recombinant viral genome, a first I-SceI recognition site located 5' to the viral genome, and a second I-SceI recognition site located 3' to the adenovirus genome into a host cell. The host cell is then cultured under conditions in which it expresses I-SceI endonuclease. The endonuclease cleaves the I-SceI recognition sites, thereby rescuing the recombinant virus from the plasmid, making it available for packaging into an infectious virus.