发明名称 |
A PROCESS OF LABELING PROTEIN BY USING WHEAT EMBRYO CELL-FREE PROTEIN SYNTHESIS SYSTEM |
摘要 |
Utilizing a what embryo cell-free protein synthesis system, there are provided a process for the production of selenomethionine-labeled protein, characterized in that, methionine in a wheat embryo extract for a cell-free protein synthesis obtained by a complete removal of endosperm contaminated is changed to selenomethionine and a cell-free protein synthesis is carried out using a reaction solution composition for protein synthesis containing selenomethionine instead of methionine under a batch condition or a dialysis condition and also the said protein produced as such. There are further provided a process for the production of heavy hydrogen-labeled protein using the same means and also the said protein produced as such.
|
申请公布号 |
EP1310564(A1) |
申请公布日期 |
2003.05.14 |
申请号 |
EP20010951912 |
申请日期 |
2001.07.18 |
申请人 |
NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY;WAKENYAKU CO., LTD.;ENDO, YAETA, DR. |
发明人 |
ENDO, YAETA;KUMAR, PENMETCHA;NISHIKAWA, SHIGEMICHI |
分类号 |
C12N15/10;(IPC1-7):C07K1/13;C12N15/09;C12P21/02 |
主分类号 |
C12N15/10 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|