METHOD FOR PRODUCING BOVINE TUMOR NECROSIS FACTOR

摘要

PROBLEM TO BE SOLVED: To provide a method for producing a highly purified bovine tumor necrosis factor (bTNF-α) in large scale. SOLUTION: The bovine tumor necrosis factor is formed, accumulated in a culture preparation by culturing Brevibacillus choshinensis transformed by a plasmid incorporated with a DNA fragment having a base sequence encoding an amino acid sequence of a matured bovine tumor necrosis factor-α to collect it.