NONHUMAN MODEL ANIMAL OF TH2-MEDIATED HYPERIMMUNE RESPONSE

摘要

It is intended to provide a nonhuman model animal of Th2-mediated hyperimmun e response lacking PIR-B gene function on chromosome by which the Th2-mediated immune response mechanism and allergy onset mechanism in vivo can be analyze d and which is liable to suffer from not only hyper-response of B cells but al so allergy, and an inducer/promoter or an inhibitor for Th2-mediated immune response, etc. with the use of the nonhuman model animal of Th2-mediated hyperimmune response. The nonhuman model animal of Th2-mediated hyperimmune response is prepared by integrating a fragment comprising exons 1 to 7 and t he domain in the 5~ side of exon 8 of mouse PIR-B gene and another fragment containing exons 10 to 14 into a vector pMC1-Neo, cleaving it with Xho I-Sal I, integrating it into a vector pIC19R-MC1tk having herpes virus thymidine kina se to thereby construct targeting vector, transferring the targeting vector int o ES cells and then injecting the ES cells into blastocyst.