A recombinant microorganism comprises an asporogenic Bacillus subtilis strain in which a gene encoding a protease enzyme has been downregulated or inactivated. In particular sigma factor spoIIAC is inactivated such that the strain is asporogenic. These strains are particularly useful as expression vehicles for proteins such as protective antigen (PA) of Bacillus anthracis.
申请公布号
WO03006649(A3)
申请公布日期
2003.05.08
申请号
WO2002GB03166
申请日期
2002.07.09
申请人
THE SECRETARY OF STATE FOR DEFENCE;WILLIAMSON, ETHEL, DIANE;BAILLIE, LESLIE, WILLIAM, JAMES;MILLER, JULIE