METHOD OF INDUCING DIFFERENTIATION OF MESOBLAST STEM CELLS OR ES CELLS INTO NERVE SYSTEM CELLS

摘要

It is found out that when mesoblast stem cells or ES cells prepared from a mononuclear fraction separated from bone marrow fluid or cord blood are cultured in a fundamental liquid culture medium, the differentiation of these mesoblast stem cells or ES cells into nerve system cells or glial cells is induced. It is further found out that the induction of the differentiation of mesoblast stem cells or ES cells into nerve system cells is promoted by adding an ischemic brain extract to the above-described fundamental liquid culture medium. It is clarified that the nerve system cells obtained by the above method are capable of regenerating nerve in a cerebral infarction model, a dementia model, a spinal injury model and a demyelination model.