| 摘要 |
A method tests a gene and includes the steps of amplifying a target region to be analyzed of a double-stranded DNA using primers and dNTPs, enzymatically decomposing pyrophosphate formed in the former step and the primers and dNTP used therein, repeatedly synthesizing a complementary strand using primers specific to the sequence and mutation of the target region to be analyzed, and converting a pyrophosphate formed in the step of synthesizing into ATP, allowing the ATP to react with a chemiluminescent reagent to generate chemiluminescence, and monitoring the generated chemiluminescence to thereby detect the presence of the target to be analyzed. This method can easily and simply be performed with high sensitivity at low cost, in which a series of reactions can be performed in a homogenous measuring system.
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