| 摘要 |
The invention concerns a method for enriching a population of keratinocyte stem cells, from a preparation of keratinocytes, comprising a pre-enrichment step in keratinocyte stem cells by adherence of said preparation on a constituent of extracellular matrices, in particular recognized by molecules involved in the adherence of the keratinocytes, in particular by the integrin family receptors, particularly collagen, for a period of time between about 5 and about 30 minutes, preferably between about 10 and about 20 minutes, advantageously 12 minutes, and in recuperating the stem cells which have adhered on said constituent of extracellular matrices by separation in conditions enabling to maintain the viabilty of the stem cells which have adhered, in particular by trypsinization, particularly using trypsin 0.05 % and EDTA 0.02 % to obtain a population of adherent cells.
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