Antibiotics-independent vector for constant high-expression and method for gene expression using the same

摘要

The present invention relates to an antibiotic-independent vector for constant high-expression and a method for gene expression using this vector. The stable expression of a recombinant gene is maintained by a stable plasmid through the use of genes essential for the growth and proliferation of the microorganism as a marker, instead of antibiotics, such as ampicillin. The application of the antibiotic-independent vector for constant high-expression in the present invention would appear to overcome the problems related to the use of high-priced inducing substances and antibiotic-resistant genes. Plus, the pure production of various beneficial DNAs and recombination proteins can be achieved.