| 摘要 |
This invention provides novel chromogenic enzyme substrates which are indoxyl beta -D-ribofuranosides. A process for their production is provided. Methods for detecting beta -D-ribofuranosidase activity are given. The advantages of these novel compounds includes: detecting beta -D-ribofuranosidase activity with high sensitivity and low substrate concentrations and use with other enzyme indicators in situations where a plurality of enzyme activities is to be visualized simultaneously e.g. for identifying bacteria growth on solid growth media. The synthesis and use of 5-bromo-4-chloro-3-indolyl- beta -D-ribofuranoside is exemplified. |
