| 摘要 |
<p>A method of detecting or determining C-reactive protein (CRP) in diverse animal species comprises contacting a sample of a biological fluid from a human or non-human animal with a complex of a phosphorylated compound containing a nitrogen moiety and a label which is directly detectable in a non-immunological assay, the phosphoryl moiety and the nitrogen moiety being positioned relative to each other so as to permit binding to calcium-dependent ligand binding sites present on CRP. The method enables one to carry out in a simple and rapid manner assays, some involving no signal amplification steps, which facilitate the non-immunological detection of CRP in a range of species including human, porcine and canine species. These assays enable the determination of the normal versus disease-state where elevated CRP levels are present.</p> |
