发明名称 |
NUCLEIC ACID EXTRACTION METHOD AND KIT |
摘要 |
The present invention relates to a method and a kit for isolating nucleic acids from tissue samples. In particular, but not exclusively, formalin fixed paraffin embedded tissue samples. The isolated nucleic acids will normally be for use in a polymerase chain reaction (PCR). The inventive method comprises the sequential steps of: a) placing in a container the tissue sample, and a wax, a detergent and a tissue digestion enzyme; b) heating the container to a first temperature; c) raising the temperature of the container to a second temperature; d) cooling the container; e) forming a waxy-layer containing impurities and a nucleic acid containing layer; f) removing the waxy-layer, such that the nucleic acids are usable in a polymerase chain reaction. Kits are also provided for performing the same.
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申请公布号 |
WO0246463(A9) |
申请公布日期 |
2003.05.01 |
申请号 |
WO2001GB05370 |
申请日期 |
2001.12.04 |
申请人 |
GENOVAR DIAGNOSTICS LTD;SMITH, CHRISTOPHER, ALBERT, DALE;COOMBS, NATHAN |
发明人 |
SMITH, CHRISTOPHER, ALBERT, DALE;COOMBS, NATHAN |
分类号 |
C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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