TARGET SPECIFIC SCREENING ANDITS USE FOR IDENTIFYING TARGET BINDERS

摘要

The present invention relates to a modified phage display method for detecting and identifying target and target binders. The modified methods involve transforming host cells with two separate phages, one comprising a target or target library and the other comprising a target-binder library, and selecting to eliminate the non-paired targets and binders. Also related are complexes comprising target and target binders, as well as antibodies that bind to these complexes. Additionally, the present invention also relates to nucleic acids encoding target and target binders peptides. The invention also relates to the use of the sequence information inherent in the targets and target binders for target validation using in silico approaches. In addition, the invention also relates to diagnostics and therapeutics employing the disclosed target and target binder pepetides or polynucleotides and their use in small molecule drug discovery. In particular, the present invention relates to diagnostic and therapeutic applications directed to cancers and similar disorders and the isolation of small molecule drugs by using the target and target binders to generate site directed assays for high throughput drug screening.