Sialyltransferase and DNA encoding the same

摘要

A sialyltransferase having the following physico-chemical properties:(1) Activity:transfers sialic acid from a sialic acid donor selectively to a 3-hydroxyl group of a galactose residue contained in lactosylceramide as a sialic acid acceptor to produce ganglioside GM3;(2) Optimal reaction pH:6.0 to 7.0; and(3) Inhibition and activation:the activity increases at least 1.5 times with 10 mM of Mn2+ as compared with the case in the absence thereof.