Process for production of mammalian cell lines

摘要

Described is a process for generating a mammalian cell line from primary mammalian cells, comprising the step of:a) pre-treating a culture of said primary mammalian cells or a suspension thereof with at least one glucocorticoid,b) optional step comprising obtaining a suspension of said pre-treated culture of step a),c) transferring into the pre-treated cells of the suspension of step a) or b) at least one nucleic acid vector which is not of retroviral origin and which is competent to immortalize said pre-treated cells andd) culturing the transferred cells of step c).Furthermore, mammalian cell lines and cells obtainable by the described process are provided as well as pharmaceutical and diagnostic compositions containing such cells.