| 摘要 |
A bioreactor is described comprising: at least one cartridge having disposed therein at least one collagen substrate comprised of a collagen sponge layer and a nonporous to cells, semipermeable collagen layer; a substrate support that retains the collagen substrate within the cartridge; a first compartment between the collagen sponge layer and the inner surface of the first side of the cartridge casing and a second compartment between the nonporous to cells, semipermeable collagen layer and the inner surface of the second side of the cartridge casing; inlet and outlet means for transferring a first medium and a first cell type to the first compartment and inlet and outlet means for transferring a second medium and a second cell type to the second compartment. Also described is a method for using the bioreactor to grow a Composite Living Construct (CLC) comprised of a first layer comprising a cultured first cell type and a second layer comprising a cultured second cell type; seeding and culturing the first cell type on and within the collagen sponge layer; and seeding and culturing the second cell type on the nonporous to cells, semipermeable collagen layer. Another embodiment of this invention includes methods for cutting the CLC into sections or units in preparation for product packaging of appropriate sizes. Yet another embodiment of this invention includes equilibration of the CLC with cryoprotectant solutions within the cartridge, further cutting the CLC into sections or units in preparation for cryopreservation. Yet another embodiment of this invention includes equilibration of the CLC with cryoprotectant solutions and cryopreservation within the cartridge, with the cartridge providing product packaging. Yet another embodiment of this invention includes methods for cutting the CLC into sections or units in preparation for product packaging, followed by equilibration with cryoprotectant solutions and cryopreservation within the package.
|
