发明名称 |
METHOD FOR PREPARATION OF SINGLE CHAIN ANTIBODIES |
摘要 |
This invention provides a method for identifying cells expressing a target single chain antibody (scFv) directed against a target antigen from a collection of cells that includes cells that do not express the target scFv, comprising the step of combining the collection of cells with an anti- idiotype directed to an antibody specific for the target antigen and detecting interaction, if any, of the anti-idiotype with the cells, wherein the Occurrence of an interaction identifies the cell as one which expresses the target scFv. This invention also provides a method for making a single chain antibody (scFv) directed against an antigen, wherein the 15 selection of clones is made based upon interaction of those clones with an appropriate anti-idiotype, and heretofore inaccessible scFv so made. This invention provides the above , methods or any combination thereof. Finally, this invention! provides various uses of these methods. |
申请公布号 |
WO03033670(A2) |
申请公布日期 |
2003.04.24 |
申请号 |
WO2002US33331 |
申请日期 |
2002.10.17 |
申请人 |
SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH;CHEUNG, NAI-KONG, V.;GUO, HONG-FEN |
发明人 |
CHEUNG, NAI-KONG, V.;GUO, HONG-FEN |
分类号 |
A61K6/00;A61K39/395;A61K47/48;A61K49/00;A61P43/00;C07K16/00;C07K16/18;C07K16/30;C07K16/42;C12N15/11;C12N15/63;C12N15/85;C12Q1/68;C12Q1/70;G01N33/00;G01N33/53 |
主分类号 |
A61K6/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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