| 摘要 |
A method to improve neural cell viability in brain or spinal cord tissue after brain or spinal cord injury or surgery is provided. This method comprises applying a sterile liquid medium to the brain or spinal cord tissue, wherein the sterile aqueous liquid medium comprises 0 to about 3000 muM CaCl2, about 0.1 to about 1.2 muM Fe(NO3)3, about 2500 to about 10000 muM KCl, 0 to about 4000 muM MgCl2, about 30000 to about 150000 muM NaCl, about 100 to about 30000 muM NaHCO3, about 250 to about 4000 muM NaH2PO4, about 0.01 to about 0.4 muM sodium selenite, about 0.2 to about 2 muM ZnSO4, about 2500 to about 50000 muM D-glucose, about 1 to about 50 muM L-carnitine, about 3 to about 80 muM ethanolamine, about 15 to about 400 muM D(+)-galactose, about 40 to about 800 muM putrescine, about 20 to about 500 muM sodium pyruvate, and growth-promoting essential fatty acids, hormones, amino acids, vitamins and anti-oxidants in amounts effective for neuron growth, and wherein the medium is essentially free of ferrous sulfate, glutamate, and aspartate.
|
