CLONING AND RECOMBINANT EXPRESSION OF MAMMALIAN SECRETED GROUP IIF PHOPHOLIPASE A2

摘要

The present invention concerns DNA and peptide sequences encoding a novel mammalian secreted group IIF phospholipase A2 wherein said enzyme is Ca<2+>-dependent, maximally active at pH 7-8, and hydrolyzes phosphatidylglycerol versus phosphatidylcholine with a 15-fold preference and more particularly, a novel human group IIF phospholipase A2. The invention also concerns the use of this enzyme in methods for screening various chemical compounds.