Identifying recombinant nucleic acids in a mixture, useful for detecting fused gene segments, comprises incomplete hybridization with a reference mixture

摘要

Identifying a recombinant nucleic acid (A) in a nucleic acid mixture, comprising: (a) preparing a nucleic acid mixture (B1) for testing and a second comparison mixture (B2) able to hybridize to B1 but lacking (A); (b) combining the two mixtures so that hybridization occurs; and (c) separating the nucleic acid in which the 5'-end is single stranded, is new.