发明名称 |
SINGLE MOLECULE SEQUENCING METHOD |
摘要 |
The invention relates to a method for single molecule sequencing of nucleic acids that comprises the following steps: a) providing a support particle with a nucleic acid molecule immobilized thereon, substantially all nucleotide components of at least one base type in at least one strand of the nucleic acid molecule carrying a fluorescent label, b) introducing the support particle into a microchannel sequencing device, c) retaining the support particle in the sequencing device, d) progressively cleaving individual nucleotide components from the immobilized nucleic acid molecule, e) guiding the cleaved off nucleotide components through a microchannel by means of a hydrodynamic flow, and f) identifying the base sequence of the nucleic acid molecule by the order of the cleaved off nucleotide components. The invention also relates to a device for carrying out the inventive method. |
申请公布号 |
WO0202225(A3) |
申请公布日期 |
2003.04.24 |
申请号 |
WO2001EP07460 |
申请日期 |
2001.06.29 |
申请人 |
GNOTHIS HOLDING SA;FOELDES-PAPP, ZENO;HOLM, JOHAN |
发明人 |
FOELDES-PAPP, ZENO;HOLM, JOHAN |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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