Compositions and methods for recombinational cloning of nucleic acid molecules

摘要

The present invention relates generally to recombinant DNA technology. The invention relates more specifically to compositions and methods for recombinational cloning of nucleic acid molecules using recombination systems. In particular, the invention relates to compositions comprising one or more Fis proteins and one or more additional components used for recombinational cloning (such as one or more recombination proteins). The invention further relates to the use of the above compositions in methods of recombinational cloning of nucleic acid molecules. The invention also relates to isolated nucleic acid molecules produced by methods of the invention, to vectors comprising such nucleic acid molecules, and to host cells comprising such nucleic acid molecules and vectors.