| 摘要 |
PROBLEM TO BE SOLVED: To provide a new method for isothermal, single primer linear nucleic acid amplification. SOLUTION: This method comprises the following steps: (a) a step of hybridizing a single-stranded DNA template comprising a target sequence with a composite primer comprising an RNA portion and a 3' DNA portion, (b) a step of optionally hybridizing a polynucleotide comprising a termination polynucleotide sequence to a region of the template which is on the 5' side with respect to hybridization of the composite primer to the template, (c) a step of extending the composite primer with a DNA polymerase, and (d) a step of cleaving the RNA portion of the annealed composite primer with an enzyme that cleaves the RNA from an RNA/DNA hybrid such that another composite primer is capable of hydridizing to the template and repeating primer extension by strand displacement. |
