| 摘要 |
<p>The present invention relates to a method of analysing protein and/or peptide expression, which method comprises the steps of (a) providing a sample comprised of disintegrated cells; (b) treating the sample with a protecting agent so as to block epsilon amino groups present on lysine residues of proteins present therein; (c) cleaving the proteins into a peptide mixture; (e) treating at least partially separated peptides with a labelled agent which binds directly to N-terminal amino acids of peptides; and (d) at least partially separating the labelled peptides.</p> |
