发明名称 |
ANTIBODY COMPLEXES AND METHODS FOR IMMUNOLABELING |
摘要 |
The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno- labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-F c Fab or Fab' fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno- labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeli ng reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.
|
申请公布号 |
CA2462280(A1) |
申请公布日期 |
2003.04.17 |
申请号 |
CA20022462280 |
申请日期 |
2002.10.02 |
申请人 |
MOLECULAR PROBES, INC. |
发明人 |
ARCHER, ROBERT M.;HAGEN, DAVID C.;HAUGLAND, ROSARIA P.;BEECHEM, JOSEPH M.;HAUGLAND, RICHARD P. |
分类号 |
G01N33/53;G01N33/58;G01N33/68;(IPC1-7):G01N33/58 |
主分类号 |
G01N33/53 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|