发明名称 Method and apparatus for maintenance and expansion of hemopoietic stem cells and/or progenitor cells
摘要 The present invention concerns a method of preparing a conditioned medium from a confluent stromal cell culture, the method comprising: (a) culturing stromal cells to confluence in a stationary phase plug-flow bioreactor under continuous flow of medium on a three dimensional substrate to obtain a stationary phase three-dimensional confluent stromal cell culture, wherein said stromal cell culture is grown to a density of at least 5 x 10 6 cells per a cubic centimeter of said substrate; and (b) separating and collecting a stromal cell conditioned medium from said stationary phase confluent stromal cell culture; thereby obtaining the conditioned medium.
申请公布号 AU759719(B2) 申请公布日期 2003.04.17
申请号 AU20000034807 申请日期 2000.02.04
申请人 TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LTD. 发明人 SHOSHANA MERCHAV;SHAI MERETSKI
分类号 C12M3/00;A61F2/00;A61K35/12;A61K35/14;A61P7/00;A61P43/00;C12M3/04;C12N5/00;C12N5/02;C12N5/06;C12N5/078;C12N5/08;C12N11/08;C12R1/91;(IPC1-7):C12N5/00;A61F2/02;C12N11/02 主分类号 C12M3/00
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