New telomerase-immortalized cell line, useful e.g. for examining the effects of xenobiotics, is produced by transfection of human endometrial cells

摘要

New telomerase-immortalized cell line (A), produced from a human endometrial cell, isolated from uterine tissue in the proliferation phase of the cycle, and transfected with the eukaryotic expression plasmid pCI-neo-hTERT (= human telomerase reverse transcriptase) or pcDNA3-hTERTn2. Stable transfectants, designated hTERT-EE, are then isolated by geneticin selection.