The present invention provides a novel helper phage and phagemid and phagemid display system that comprises an amber mutation in gene 3 of the helper phage so that it is not expressed in the non-permissive bacteria and an in-frame stop codon in the phagemid prior to the gene 3 coding sequence that prevents expression of g3p unless a foreign gene is inserted therein, thus preventing propagation of insert-less phagemids. This results in improved display of foreign gene products on individual virions, avoidance of virions lacking foreign gene inserts and the creation of large phage display libraries.
申请公布号
WO03031611(A2)
申请公布日期
2003.04.17
申请号
WO2002CA01496
申请日期
2002.10.04
申请人
CANGENE CORPORATION;WIERSMA, ERIK, JOHAN;STEWART, DONALD, IAN, HALL