| 摘要 |
The present invention provides a diagnostic method for congenital adrenal hyperplasia, comprising the steps of: preparing oligonucleotide chip in which 7 sets of tandem cDNA fragments of normal and patient's 21-hydroxylase gene (exon 1, intron 2, exon 4) are immobilized to solid support; amplifying the DNA segments of 21-hydroxylase gene (exon 1, intron 2, exon 4) from the genomnic DNA of a testee using labeled primers under the suitable condition for carrying out polymerase chain reaction (PCR); hybridizing 7 sets of tandem cDNA fragments in oligonucleotide chip with the PCR product by amplifying gDNA segments; and analyzing the results of the hybridization.
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