METHOD FOR PREPARING COBALT-PROTEIN COMPLEX

摘要

A method for preparing a cobalt−apoferritin complex which comprises a first step (St1 in Fig.2) of mixing a HEPES buffer solution, an apoferritin solution and a Co2+ ion solution (for example, cobalt nitrate solution) in this order, to prepare a reaction solution, and a second step (St2) of adding an oxidizing agent (for example, H2O2) to the reaction solution, to thereby introduce cobalt hydroxide (CoO(OH)) into the holding sector (4) of apoferritin (1) and form a cobalt−apoferritin complex. The above operations for forming a cobalt−apoferritin complex are carried out while agitating with a stirrer at room temperature. The method allows the preparation of a cobalt−apoferritin complex including fine cobalt particles having a uniform particle diameter.