发明名称 |
Method for recognizing bacterial DNA |
摘要 |
<p>The present invention provides a receptor protein specifically recognizing bacterial DNA having an unmethylated CpG sequence, a genomic DNA encoding it, an experimental animal model useful for examining responsiveness of a host immune cell against a bacterial infectious disease. DNA encoding a receptor protein specifically recognizing bacterial DNA having an unmethylated CpG sequence is screened by BLAST search, a number of EST clones having high homology with various TLRs is screened, these clones are used as a probe to isolate a full-length cDNA from mouse macrophage cDNA library, and the sequence of bases of the cDNA is analyzed to confirm that it is TLR9 comprising a conserved regions such as LRR and TIR regions, and then a knockout mouse is produced to confirm that TLR9 is a receptor protein of oligonucleotides having an unmethylated CpG sequence of bacterial DNA. <IMAGE></p> |
申请公布号 |
EP1302541(A1) |
申请公布日期 |
2003.04.16 |
申请号 |
EP20010934545 |
申请日期 |
2001.06.05 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY CORPORATION |
发明人 |
AKIRA, SHIZUO;HEMMI, HIROAKI |
分类号 |
G01N33/50;A61K38/00;A61K45/00;A61P31/04;A61P35/00;A61P37/08;C07K14/005;C07K14/705;C07K16/28;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/09;C12N15/12;C12P21/08;C12Q1/68;C12R1/91;G01N33/15;G01N33/566;G01N33/577;(IPC1-7):C12N15/12;C12P21/02 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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