发明名称 |
A LIBRARY OF A COLLECTION OF CELLS |
摘要 |
The present invention relates to combinatorial gene expression libraries and methods for making these. The library comprises a collection of individual cells, the cells being denoted celll,cell2..., cell, wherein i > 2, each cell comprising at least one concatemer of individual oligonucleotide cassettes, each concatemer comprising a nucleotide sequence of the following formula: (rs2-SP-PR-X-TR-SP-rs1) n wherein rsl and rs2 together denote arestriction site, SP denotes aspacerof at least two bases, X denotes anexpressible nucleotide sequence,PRdenotes a promoter, capable of regulatingthe expression of X in the cell,TRdenotes a terminator, and n 2, and whereinat least one concatemer of celllisdifferent from a concatemer cell2.Such libraries are useful in discovery of novel and/or enhanced metabolic pathways leading to the production of novel compounds for e.g. drug discovery and/or to the production of known compounds in novel quantities or in novel compartments of the cells. The expression libraries in particular are composed of host cells capable of co-ordinated and controllable expression of large numbers of heterologous gene in the host cells. co-ordinated and controllable expression of large numbers of heterologous gene i host cells. |
申请公布号 |
WO02059297(A3) |
申请公布日期 |
2003.04.10 |
申请号 |
WO2002DK00056 |
申请日期 |
2002.01.25 |
申请人 |
EVOLVA BIOTECH A/S;GOLDSMITH, NEIL;SOERENSEN, ALEXANDRA, M., P., SANTANA;NIELSEN, SOEREN, V., S. |
发明人 |
GOLDSMITH, NEIL;SOERENSEN, ALEXANDRA, M., P., SANTANA;NIELSEN, SOEREN, V., S. |
分类号 |
C12N15/09;C12N1/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/10;C12N15/66 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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