| 摘要 |
<p>A high numerical aperture flow cytometer having a laser input (20) which emits a beam of light oriented substantially orthogonally to the flow of blood cells through a flow cell (18). A portion of the beam impinging upon the cells is scattered at a substantially right angle to the beam of laser input and is received by a right angle scatter light detector (22). A second portion of the beam impinging upon the cells in the flow cell is scattered at a much lower angle than 90° and is collected on two distinct photo detectors, that represent 'forward scatter low' which has an angle of from about 1° to about 3° and 'forward scatter high' which has an angle of from about 9° to about 12° from the original beam from laser input. A third photo detector is placed between the two forward scatter detectors and measures axial light loss.</p> |
