| 摘要 |
A method for generating a mutein of human apolipoprotein D having detectable affinity to a given non-natural ligand of apolipoprotein D is disclosed, whi ch comprises the steps of: (a) subjecting the apolipoprotein D to mutagenesis a t the sequence positions 34 to 38, 60, 62 to 66, 68, 89 to 93, 115, 117 to 121 , and 123 resulting in a plurality of muteins of apolipoprotein D; and (b) enriching resulting muteins having binding affinity for a given ligand from the plurality of muteins by selection, and/or isolating said mutein. Muteins of apolipoprotein D obtainable by this method are also disclosed.
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