FUNCTIONAL SITE PROFILES FOR PROTEINS AND METHODS OF MAKING AND USING THE SAME

摘要

Described are functional site profiles, and methods of making and using the same. Specifically, the methods employ functional site profiling to examine the chemistry and structure of functional sites in proteins. Such methods allow classification of proteins, and protein relatedness, based on functional site chemistry and structure, rather than sequence similarity or structural homology across complete protein sequences and structures. Functional site profiling of amino acid residues located in the spatial environment of key functional features allows conserved similarities and characteristic differences in functional sites across protein families to be assessed, which has application not only in terms of protein classification, but also in the context of drug discovery, protein engineering, etc.