发明名称 |
TARGET ASSISTED ITERATIVE SCREENING (TAIS) : A NOVEL SCREENING FORMAT FOR LARGE MOLECULAR REPERTOIRES |
摘要 |
This invention provides a new in vitro screening method for the detection of protein-protein and other interactions. The method has been developed and applied to a commercial cDNA library to search for novel protein-protein interactions. PDZ, WW and SH3 domains from PSD95, Nedd4, Src, Abl and Crk proteins were used as test targets. 12 novel putative and 2 previously reported interactions were identified for 6 protein interaction modules in test screens. The novel screening format, dubbed TAIS (target-assisted iterative screening), provides an alternative platform to existing technologies for a pair-wise characterization of protein-protein, and other, interactions.
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申请公布号 |
WO03029821(A1) |
申请公布日期 |
2003.04.10 |
申请号 |
WO2002US31349 |
申请日期 |
2002.10.01 |
申请人 |
BUCK INSTITUTE;KOURAKINE, ALEXEI;BREDESEN, DALE |
发明人 |
KOURAKINE, ALEXEI;BREDESEN, DALE |
分类号 |
C12N15/10;C12P21/04;C12Q1/68;C40B30/04;C40B40/02;G01N33/68;(IPC1-7):G01N33/53;C12Q1/70;C07K16/00 |
主分类号 |
C12N15/10 |
代理机构 |
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