ATTACHMENT OF THIOPHOSPHATE TETHERED OLIGONUCLEOTIDES TO A SOLID SURFACE

摘要

The present invention provides an alternative method of attaching a 3'-thiophosphate mononucleotide or oligonucleotide to a solid support. The method permits chemoselective binding of a thiophosphate mono- or oligonucleotide to a solid support. The present invention is useful in production of microarrays, chips, beads or other solid matrices for gene expression profiling, single nucleotide polymorphism, and pharmacogenomics, target validation, sequencing and for any application that involves contacting a target nucleic acid sequence with a support-bound probe.